The enzymic hydrolysis of steroid conjugates. 1. Sulphatase and β-glucuronidase activity of molluscan extracts
نویسندگان
چکیده
منابع مشابه
Steroid Sulphatase, Arylsulphatase and ,b-glucuronidase in Marine Invertebrates
The widespread occurrence of sulphatases in the Mollusca was first demonstrated by Soda & Hattori (1933a, b) who showed that these enzymes are mainly concentrated in the digestive glands. That extracts from marine molluscs also possess ,B-glucuronidase activity was first noted by Dodgson, Lewis & Spencer (1952) who studied the optimum conditions for the activity ofthis enzyme and arylsulphatase...
متن کاملdetection of volatile compounds of medicinal plants with some nano-sorbents using modified or new methodologies and investigation of antioxidant activity of their methanolic extracts
in this work, a novel and fast method for direct analysis of volatile compounds (davc) of medicinal plants has been developed by holding a filament from different parts of a plant in the gc injection port. the extraction and analysis of volatile components of a small amount of plant were carried out in one-step without any sample preparation. after optimization of temperature, extraction time a...
Hydrolysis of Conjugates of Urinary Corticoids with /3-glucuronidase
Since urinary steroids are excreted as conjugates, principally of sulfuric and glucuronic acids, hydrolysis is a necessary preliminary procedure which must be carried out before the steroids may be readily extracted by means of organic solvents. The effectiveness of bacterial action in the hydrolysis of conjugates of estrogens was demonstrated by Cohen and Marrian (1) who allowed putrefaction t...
متن کاملHydrolysis of Conjugates of Urinary Corticoids with P-glucuronidase
Hydrolysis of conjugates of urinary neutral reducing lipides by means of /?-glucuronidase of bacterial origin, as described in Paper I of this series (I), permits the extraction from urine of much larger amounts of these substances than may be obtained by extraction of acidified urine in separatory funnels. The isolation of a crystalline reducing steroid from normal urine which had been treated...
متن کاملThe hydrolysis of steroid glucuronides with calf spleen glucuronidase.
It has long been recognized that the relatively severe acid hydrolysis necessary to release steroids from their conjugates in urine may result in considerable losses or transformations (3, 4). The isolation of steroid glucuronides (5-7) and of steroid sulfates (8, 9) from human urines has led to the belief that most, if not all, of the urinary steroid conjugates exist as glucuronides or as sulf...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1956
ISSN: 0306-3283
DOI: 10.1042/bj0630705